| 稿件编号： | L3794 |
| 稿件标题： | XE-5000血细胞分析仪血小板计数性能评价 |
| 稿件作者： | 罗丽贞; |
| 关键字词： | Sysmex XE-5000；血小板计数；性能评价；全自动血细胞分析仪 |
| 文章摘要： | 【摘要】目的 评价Sysmex XE-5000血细胞分析仪对血小板（platelet PLT）的检测性能。方法 对XE-5000分别用电阻抗法和荧光法对血小板计数进行精密度，线性测试及干扰试验进行评估，并与显微镜计数法作对比试验。运用spass12.0对数据进行分析。结果 平均批内精密度为PLT-I:1.30%、PLT-O:1.40%；批间精密度为PLT-I: 1.87%、PLT-O:2.39%；携带污染率范围为PLT-I: 0～1.32%、PLT-O:0～1.17%；平均为PLT-I: 0.44%和PLT-O: 0.33%；在线性稀释实验中低、中、高值与理论值的相关系数r分别为，PLT-I: 0.991、0.997、0.997，PLT-O: 0.997、0.998、0.992；在RBC 碎片干扰实验中,PLT-O对低值样本显示出较强的抗干扰能力(t=1.14, P>0.05)，PLT-I与PLT-O测定血小板结果与PLT-M结果相关性良好。结论 Symex XE-5000对血小板计数具有精密度好，抗干扰能力强，重复性好，检测速度快等优点，是常规实验室测定血小板的较理想仪器。 |
| 杂志名称： | 检验医学与临床 |
| 文章刊期： | 2011年08卷04期 |
| 文章栏目： | 论著 |
| 稿件标题（英文）： | XE-5000 hematology analyzer performance evaluation of platelet count |
| 稿件作者（英文）： | LuoLiZHen; |
| 关键字词（英文）： | Sysmex XE-5000；platelet count；performance assessment； |
| 文章摘要（英文）： | 【Abstract】 Objective: To evaluate the platelet (PLT) count performance quality of Sysmex XE-5000 automatic hematology analyzer. Methods: PLT was counted by Sysmex XE-5000 automatic hematology analyzer with electric impedance (PLT-I) and optical (PLT-O) methods to test the precision, linear range and interference. The count results of PLT-I and PLT-O were compared with those of microscopic count method (PLT-M) respectively. All data were analyzed by SPSS 12.0. Results：The within-batch precisions of PLT-I and PLT-O were 1.30% and 1.40% and the between-batch precisions were 1.87% and 2.39%. The ranges of carry-over ratio are from 0 to 1.32% with PLT-I and from 0 to 1.17% with PLT-O,the average carry-over ratio of PLT-I and PLT-O were 0.44% and 0.33%. The correlation coeffients (r)were 0.991, 0.997, 0.997 with PLT-I and 0.997, 0.998, 0.992 with PLT-O in lower,medium and high value specimens. The result showed that the PLT-O method had great ability of anti-interference in RBC fragment interference experiment (t=1.14,p>0.05).There were significant correlations in the results between PLT-I and PLT-M and between PLT-O and PLT-M. Conclusion: Sysmex XE- 5000 automatic hematology analyzer is of high accuracy, good repeatability, quickness and anti- interference for platelet count. it is satisfied with needs of blood platelet count in the clinical laboratory. |
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