| 稿件编号： | L3749 |
| 稿件标题： | 实时荧光逆转录聚合酶链反应对疑似甲型H1N1流感样本的检测结果及分析 |
| 稿件作者： | 杨婵; |
| 关键字词： | 甲型H1N1流感病毒 流行特征 real time PT-PCR |
| 文章摘要： | 摘要：目的 对流感疑似病人咽拭子样本进行检测，保证患者早诊断和及时分析我州甲型H1N1疫情流行动态。方法 依据世界卫生组织2009年4月30日颁布的检测甲型H1N1流感操作规程,利用real time PT-PCR技术及7300型实时荧光定量PCR仪的SDS操作软件进行检测。结果 737份流感疑似病人咽拭子样本中，甲型H1N1流感病毒阳性率为25.5%。结论 运用real time PT-PCR检测结果显示我州甲型H1N1流感疫情具有传播快，强度大，但其病死率较低的流行特征，与全国流行规律基本一致。 |
| 杂志名称： | 检验医学与临床 |
| 文章刊期： | 2011年08卷04期 |
| 文章栏目： | 临床探讨 |
| 稿件标题（英文）： | Real-time PT-PCR technique in samples of suspected novel A ( H1N1 )Influenza detection and analysis |
| 稿件作者（英文）： | yangchan; |
| 关键字词（英文）： | novel A (H1N1 )influenza virus endemic characteristics real time PT-PCR |
| 文章摘要（英文）： | Abstract: Purpose:To ensure patients with early diagnosis , the national sentinel surveillance hospital collected throat swab samples with patients suspected for testing . Methods :Based on the detection of novel A (H1N1) influenza operational procedures，promulgated by World Health Organization, April 30, 2009，the use of real time PT-PCR operating software to detect. Results:737 throat swab samples, novel A (H1N1) influenza virus positive rate was 25.5%. Conclusion: The use of real time PT-PCR results showed that Novel A (H1N1) influenza epidemic characteristics apparent rapid spread and strength, but a lower prevalence of mortality characteristics are basically consistent with the national popular rule in our state. |
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